Journal: Scientific Reports

Article Title: Effect of Cucumis sativus on Dysfunctional 3T3-L1 Adipocytes

doi: 10.1038/s41598-019-49458-6

Figure Lengend Snippet: Superoxide induction by IL-1β in 3T3-L1 adipocytes. Mature adipocytes (day 8) were cultured for 24, 48, 60, or 72 h, either alone, with IL-1β, and/or insulin 1 μM. Microphotographs were taken in a spectrofluorometer under a 20X objective at 24 ( A ), 48 ( B ), 60 ( C ), and 72 h ( D ). The percent of DHE expression was determined ( E ) with the software MetaMorph v.6.1. Results are expressed as mean ± SD, *P ≤ 0.05 with respect to cells treated with insulin 1 μM and were analyzed by ANOVA and the post-hoc Tukey-Kramer test, n = 6.

Article Snippet: The percent of DHE expression was determined ( E ) with the software MetaMorph v.6.1.

Techniques: Cell Culture, Expressing, Software

Journal: Medical Oncology (Northwood, London, England)

Article Title: SARS-CoV-2 spike protein inhibits growth of prostate cancer: a potential role of the COVID-19 vaccine killing two birds with one stone

doi: 10.1007/s12032-021-01628-1

Figure Lengend Snippet: The effect of SARS-CoV-2 spike protein on LNCaP cell survival. Results with statistically significant ( p < 0.05) differences from the control group are indicated by asterisk (*). A Clonogenic survival assay for LNCaP cells treated with various concentrations of spike protein (SP): 20 ng/mL, 50 ng/mL, 200 ng/mL for 72 h. Control consisted of medium alone for the same timeframe. Colony numbers were quantified as a total percentage of colonies in the control group. B Evaluation of the control group and SP (50 ng/mL) using a cell proliferation kit. Results reflect two independent experiments and are expressed as the average optical density (OD) plus the standard error of the mean (SEM) between control and SP groups. C IHC PCNA staining images displaying relative intensity between the control group (medium only) and the SP group (50 ng/mL), treated for 72 h prior to staining. PCNA+ cells were counted using MetaMorph software. PCNA+ cell counting was performed using a random selection of 5–6 high-power fields from three slides. Images are at original ×400 magnification

Article Snippet: Relative staining intensity was evaluated via MetaMorph image software analysis of 3–5 randomly selected high-power fields of three slides

Techniques: Control, Clonogenic Cell Survival Assay, Staining, Software, Cell Counting, Selection

Journal: Medical Oncology (Northwood, London, England)

Article Title: SARS-CoV-2 spike protein inhibits growth of prostate cancer: a potential role of the COVID-19 vaccine killing two birds with one stone

doi: 10.1007/s12032-021-01628-1

Figure Lengend Snippet: The effect of SARS-CoV-2 SP (50 ng/mL) on expression of key pro- and anti- proliferative molecules in LNCaP cells. Statistically significant results are indicated by asterisk (*) ( p < 0.05). A Comparison of mRNA expression levels in pro- and anti-proliferative molecules in LNCaP cells. Pro-proliferative molecules included cyclin B, cyclin D, cyclin E, and CDK4. Anti-proliferative molecules included p18, p21, p27, and p53. GAPDH was used as an internal control for comparison of expression levels. Each experiment was completed in triplicate and recorded on the graphs as a mean ratio of molecule densitometric Units/GAPDH + SEM (× 100). B Evaluation of CDK4 expression in LNCaP cells via IHC staining. IHC images appear as original magnification of 400X. Relative staining intensity was evaluated via MetaMorph image software analysis of 3–5 randomly selected high-power fields of three slides

Article Snippet: Relative staining intensity was evaluated via MetaMorph image software analysis of 3–5 randomly selected high-power fields of three slides

Techniques: Expressing, Comparison, Control, Immunohistochemistry, Staining, Software

Journal: Medical Oncology (Northwood, London, England)

Article Title: SARS-CoV-2 spike protein inhibits growth of prostate cancer: a potential role of the COVID-19 vaccine killing two birds with one stone

doi: 10.1007/s12032-021-01628-1

Figure Lengend Snippet: Effect of SARS-CoV-2 SP (50 ng/mL) on LNCaP cell apoptosis. Statistically significant differences in TUNEL + cells and relative caspase-3 activity between the treatment group (SP 50 ng/mL) and control group (medium alone) are indicated with an asterisk (*) ( p < 0.05). A TUNEL staining images and TUNEL + cell percentage comparison between 50 ng/mL SP treatment and control groups. IHC images appear at original ×400 magnification. TUNEL + cells were evaluated using MetaMorph image software on 3–5 randomly selected high-power fields of 3 slides. B Caspase-3 activity of control and SP 50 ng/mL groups of LNCaP cells via caspase-3 activity kit. The experiment was performed in triplicate and results are expressed as the mean caspase-3 activity compared to the controls + SEM

Article Snippet: Relative staining intensity was evaluated via MetaMorph image software analysis of 3–5 randomly selected high-power fields of three slides

Techniques: TUNEL Assay, Activity Assay, Control, Staining, Comparison, Software

Journal: Medical Oncology (Northwood, London, England)

Article Title: SARS-CoV-2 spike protein inhibits growth of prostate cancer: a potential role of the COVID-19 vaccine killing two birds with one stone

doi: 10.1007/s12032-021-01628-1

Figure Lengend Snippet: The effect of SARS-CoV-2 SP (50 ng/mL) on expression of key pro- and anti-apoptotic molecules in LNCaP cells. Statistically significant results are indicated by asterisk (*) ( p < 0.05). A Comparison of mRNA expression levels in pro- and anti-apoptotic molecules in LNCaP cells. Pro-apoptotic molecules included Fas, FasL, TRAILR1, and TRAIL. Anti-apoptotic molecules included FLIP, Bcl-2, and survivin. GAPDH was used as an internal control for comparison of expression levels. Each experiment was completed in triplicate and recorded on the graphs as a mean ratio of molecule densitometric Units/GAPDH + SEM (× 100). B Evaluation of FasL expression in LNCaP cells via IHC staining. The IHC images appear as original ×400 magnification. Relative staining intensity was evaluated via MetaMorph image software analysis of 3–5 randomly selected high-power fields of three slides

Article Snippet: Relative staining intensity was evaluated via MetaMorph image software analysis of 3–5 randomly selected high-power fields of three slides

Techniques: Expressing, Comparison, Control, Immunohistochemistry, Staining, Software